Introduction to techniques for culturing and maintaining mammalian cells, including proper use of equipment, aseptic technique, media formulation, storage, counting and subculturing of cells. Contents include proper growth conditions, cell cycle regulation, cellular responses to DNA damage, growth patterns, viability assays, transfection and an introduction to the use of fluorescent molecules in visualization of cellular structures.
This in-person class has mandatory meetings on Mondays, Wednesdays, and Fridays at the Ocean Campus. Students will primarily complete coursework by attending in-person meetings and will also need an internet enabled device to complete some coursework in Canvas.
Recommended Preparation, BIO 11; BTEC 108A; Completion of or concurrent enrollment in: CHEM 32