ID
7257

Biotechnology Lab Techniques

An introduction to laboratory techniques needed for entry-level positions in the biotechnology industry and research laboratories. The course covers basic lab skills such as solution making, bacteria cultures, plasmid DNA and protein purification. Intended as an introductory class for the students in preparation of the more advanced biotechnology classes and/or an internship in a biotechnology lab.

Stem Cell Technology

A general introduction to the principles of stem cell biology. Topics include embryonic stem cells in early development, adult stem cells, and potential applications of stem cell culture and ethical issues involved in stem cell research. Current research methods involving cell differentiation and fluorescent technology will be presented. Emphasis on laboratory techniques including culture of mouse embryonic stem cells, analysis of stem cells by immunofluorescence and flow cytometry.

Mammalian Cell Culture

Introduction to techniques for culturing and maintaining mammalian cells, including proper use of equipment, aseptic technique, media formulation, storage, counting and subculturing of cells. Contents include proper growth conditions, cell cycle regulation, cellular responses to DNA damage, growth patterns, viability assays, transfection and an introduction to the use of fluorescent molecules in visualization of cellular structures.